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Fig. 1. Expression of vFLIP in MVECs using a dual promoter lentiviral vector. (A) The vFLIP_eGFP vector (2.) expresses vFLIP and GFP emerald (Em). The original GFP vector (1.) was used as a control. Unique restriction sites are shown. LTR, long terminal repeat; 
, packaging signal; RRE, rev-responsive element; cPPT, central polypurine tract; SFFV, spleen focus-forming virus promoter; WPRE, wood chuck posttranscriptional regulatory element; Ub, ubiquitin promoter; 
U3, deletion in U3 region (SIN vector). (B) Human dermal MVECs were either not transduced or transduced with a lentiviral vector encoding GFP alone or vFLIP and emerald GFP at a MOI of 30. 48 hours post transduction, the transduction efficiency was assessed by FACScan analysis. Live cells were gated (not shown) and expression of GFP was analysed using CellQuest software. 10,000 live cells were analysed per sample. The mean percentage of transduced MVECs was 55% for vFLIP_eGFP (s.d.=5.06) and 76.3% for GFP (s.d.=4.33) determined from three experiments. Values below the percentage transduced cells represent the mean fluorescence intensity (MFI) of the positive cells. (C) GFP expression and cell morphology measured using a BioRad confocal microscope 48 hours post transduction with the two vectors at a MOI of 
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