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Figure 2


Fig. 2. vFLIP induces the nuclear translocation of NF-{kappa}B–p65 in MVECs. Untransduced MVECs and cells transduced with a GFP or vFLIP_eGFP lentiviral vector, and cultured thereafter for 48 hours, were immunostained for p65, p52 and RelB (red). DAPI was used as a nuclear stain (blue), and GFP was used to identify lentiviral-transduced cells (green). Nuclear NF-{kappa}B subunit staining can be identified by colocalisation with DAPI in the top-right panel of each set of images, and compared in GFP-positive and -negative cells (bottom-right panels). Nuclear p65 staining was principally evident in GFP-positive cells transduced with the vFLIP_eGFP vector (arrows) but not in control cells. The inset histograms compare the intensity of nuclear staining for each NF-{kappa}B subunit in GFP-positive (green plot) and GFP-negative cells.





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