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Figure 4


Fig. 4. vFLIP rescues MVECs from anoikis assayed by annexin V and propidium iodide (PI) staining. Human dermal MVECs were either not transduced or transduced with a lentiviral vector encoding GFP alone or vFLIP and GFP. 48 hours post transduction, cells were detached from the matrix by trypsinization and either replated on control wells (Attached –PH) or anoikis was induced by plating cells on polyHEMA (PH)-coated wells for 16 hours (Detached +PH). (A) Early apoptotic events were measured by labelling cells with annexin-V–biotin and detected with red-fluorescent streptavidin. Annexin V staining is plotted against the side-scatter of cells. (B) Transduction efficiency was measured by FACScan analysis of cells expressing GFP. (C) Summary of results from annexin V and PI staining.





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