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Figure 1


Fig. 1. Galectin-3 distribution in the mouse small intestine. The distribution of galectin-3 and villin in murine small intestine sections was analysed by immunohistochemistry with polyclonal antibodies directed against galectin-3 (Alexa Fluor 546, red) and monoclonal anti-villin antibodies (Alexa Fluor 488, green). (A) Overview, showing the gradual increase in galectin-3 expression along the crypt-villus axis, whereas villin distributes uniformly at the brush border membrane. Arrowheads indicate goblet cells. Enlarged view of villus tip (B) and crypt area (C). Nuclear counterstaining with Hoechst 33342 is blue. Scale bars: 40 µm (A) and 20 µm (B,C).





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