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Figure 8


Fig. 8. Distribution of actin and villin in 3D reconstructed enterocytes from wt and gal3–/– mice. Transverse sections of small intestine of wt (A, supplementary material Movie 1) (gal3+/+) and gal3-null mutant mice (B, supplementary material Movie 2) (gal3–/–) were stained with antibodies against β-actin (Alexa Fluor 546, red) and villin (Alexa Fluor 488, green). For 3D reconstruction, 35 layers of each sample were recorded and processed by deconvolution and 3D rendering (see also supplementary material Movies 1 and 2). In A, co-staining of actin and villin in the apical brush border is indicated by arrowheads. Arrows in B indicate actin- and villin-positive structures in the lateral membranes (B). Hoechst 33342 nuclear counterstaining is blue. Scale bars: 20 µm.





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