JCS015172 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 (Adobe PDF) -

  Table. S1. This table has accidentally been labelled Table 2 in the article.

• Supplemental Figure S1 -

  Fig. S5. ROK and mDia induce the formation of different types of actin structures. HeLa cells were transfected with different cDNA constructs encoding GFP, Flag-tagged wild-type ROK and Flag-mDia-ΔGBD. The cells were fixed and immunostained with anti-Flag antibodies and rhodamine-coupled phalloidin. A duplicate set was treated with 10 µM 27632 (ROK inhibitor) for 1 hour before fixing. Cells transfected with mDia-ΔGBD showed long and thin actin filaments. These filaments were not disrupted by the use of ROK inhibitor. Arrows represent transfected cells. Star in lower-right panel represents an untransfected cell with loss of stress fibres after treatment of ROK inhibitor. Scale bar: 10 µm.