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Fig. 1. Nuclear dynamics in dyn1 ${Delta}$ / ${Delta}$ hyphae. Time-lapse microscopy was performed as described in Materials and Methods. Images were acquired at 5-minute intervals. Time, minutes. (A) Spindle dynamics in wild-type hyphae. The bipolar spindle moves into the germ tube (56 minutes) before anaphase (101 minutes). The septum becomes evident after spindle disassembly (arrow, 114 minutes). (B) In a dyn1 ${Delta}$ / ${Delta}$ hypha, a bipolar spindle moves into the germ tube (47 minutes) and reaches the presumptum (96 minutes) before anaphase (106 minutes). The septum is evident after spindle disassembly (arrow, 121 minutes). (C) Spindle migration fails in a dyn1 ${Delta}$ / ${Delta}$ hypha. Anaphase begins with the bipolar spindle in the basal cell (109 minutes). The dSPB does not cross the presumptum before septation (167 minutes). After septation, the hyphal tip swelled slightly before elongation arrested. Arrowheads, SPBs after septation. Bar, 10 µm.

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