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Figure 2


Fig. 2. MT dynamics during spindle movement in dyn1{Delta}/{Delta} hyphae. (A) A bipolar spindle in a wild-type hypha migrates into the germ tube (angled line) even as the dSPB-bound MT grows longer (arrowheads). Time, seconds. (B) A bipolar spindle in a dyn1{Delta}/{Delta} hypha remains at the bud neck (straight line) despite repeated MT growth, shrinkage and interaction with the cortex. Arrows, SPBs; arrowheads, MT plus-ends; time, seconds. (C) Bipolar spindle movement captured in a dyn1{Delta}/{Delta} hypha at 15-second intervals. Time, minutes. Bar, 10 µm. (D) An example of spindle position (y-axis) plotted against time (x-axis) used to indirectly calculate rates of spindle movement in dyn1{Delta}/{Delta} hyphae (black triangles). An example of spindle position versus time in a wild-type hypha is also shown for comparison (grey squares).





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