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Figure 3


Fig. 3. Dynein contributes to nuclear migration during cell cycle arrest. (A) Wild-type (YJB6284) and dyn1{Delta}/{Delta} (YJB7812) strains were grown to stationary phase overnight in YPD at 30°C. They were then diluted 1:100 into fresh YPD containing 100 mM HU and grown at 30°C for 6 hours before being fixed with ethanol and stained with DAPI for imaging. Bar, 10 µm. (B) Scatter plot of nuclear position with respect to the bud tip (y-axis) plotted against bud length (x-axis). Strains: wild-type, BWP17; dyn1{Delta}/{Delta}, YJB7812.





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