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Figure 4


Fig. 4. Colony and cellular morphology of dynein-dynactin mutants. (A-C) Colony morphology of wild-type (A), dyn1{Delta}/{Delta} (B) and nip100{Delta}/{Delta} (C) colonies after 2 days of growth on YPD-agar at 30°C. (D-F) Cellular morphology of wild-type (D), dyn1{Delta}/{Delta} (E) and nip100{Delta}/{Delta} (F) cells diluted to OD600=1.0 in YPD liquid medium and grown at 30°C for 4 hours. Cells were collected, fixed with ethanol and stained with DAPI for simultaneous DIC-DAPI microscopy. Arrowheads, yeast cells with a single nucleus; arrows, binucleate cells; carets, anucleate cells. Bar, 10 µm.





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