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Fig. 7. Bub2p mediates cell cycle progression and morphogenesis in dynein-depleted hyphae. Strains were grown to stationary phase overnight at 30°C in either PMET3 ON or PMET3 OFF conditions and then diluted 1:20 into the same medium containing 10% serum and prewarmed to 37°C. They were grown at 37°C for 2 hours, fixed with ethanol and stained with DAPI. Strains: wild-type, YJB6284 (Bensen et al., 2002), PMET3-DYN1/dyn1
, YJB10155, bub2/, CB432 (Bachewich et al., 2005), PMET3-DYN1/dyn1 bub2/, YJB9993.
