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Figure 4


Fig. 4. ER stress induced by TH or TN inhibits thyroid-specific gene expression, at least in part, at the transcriptional level. (A) Relative luciferase activity of extracts of PC Cl3 cells transfected in triplicate with 2.5 µg NISLUC2 luciferase reporter plasmid. 24 hours after transfection, cells were vehicle treated (`C') or treated with 0.5 µM TH or 0.5 µg/ml TN for 30 minutes and harvested after 24 hours in medium without TH or TN. Measurements were normalized for β-galactosidase activity driven by a co-transfected plasmid encoding RSV-βgal. Values shown (in arbitrary units) represent the mean (±s.d.) of at least three independent experiments. (B) Run-on assay performed on nuclei prepared from PC Cl3 cells vehicle treated (`C') or treated with 0.5 µg/ml TN for 30 minutes, followed by 24 hours in medium without TN. Pax8 and GAPDH probes were immobilized on filters.





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