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Fig. 7. ER stress induces an EMT-like phenotype in FRT cells. (A) Northern blot analysis of total RNA extracted from FRT cells vehicle treated or treated with increasing concentrations of TH and TN for 30 minutes, followed by 24 hours in medium without TH or TN. (B) FRT cells were grown on glass coverslips for 48 hours, then were vehicle treated or treated with 0.5 µg/ml TN for 30 minutes, followed by 24 hours in medium without TN. Cells were stained with antibodies against E-cadherin and rhodamine-conjugated phalloidin. Following TN treatment, the signal for E-cadherin decreased and stress fibers were formed. Bars, 20 µm. (C) RT-PCR analysis of total RNA extracted from FRT cells vehicle treated or treated with 0.5 µM TH or 0.5 µg/ml TN for 30 minutes, followed by 24 and 48 hours in medium without TH or TN.
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