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Figure 8


Fig. 8. TN decreases transepithelial resistance in FRT cells. (A) 2x106 cells were plated on 24.5 mm diameter Transwell filters. At confluency, transepithelial resistance was measured every 12 hours, until a plateau was reached (3-4 days). Then, cells were vehicle treated (`C') or treated with 0.5 µg/ml TN in the inferior chamber (TN inf) or in inferior plus superior chambers (TN inf + sup), and transepithelial resistance measured every 12 hours over a 4 day period. (B) FRT cells, vehicle treated and treated as in A, were observed daily by light microscopy to verify the integrity of the monolayer. At day four (last transepithelial resistance measurement), the cells were photographed.





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