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Fig. 9. c-Src is involved in ER stress-induced thyroid dedifferentiation. (A) Northern blot analysis of total RNA extracted from PC Cl3 cells pretreated or not for 30 minutes with 5, 10 or 20 µM PP2, then vehicle treated or treated for 30 minutes with 0.5 µM TH or 0.5 µg/ml TN in the presence or absence of PP2, followed by 24 hours in medium without TH or TN, but with PP2. The same filter was probed with Pax8 and BiP. (B) RT-PCR analysis of total RNA extracted from PC Cl3 cells treated as in A. (C) Western blots of total protein extracts from PC Cl3 cells starved for 48 hours in hormone- and serum-free medium, pretreated with 5 µM PP2, then vehicle treated or treated for 30 minutes with 0.5 or 1.0 µM TH or 0.5 or 1 µg/ml TN in the absence or presence of 5 µM PP2. Filters were probed with antibodies against phosphorylated Src (p-Src; Tyr416) or total Src.
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