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Figure 1


Fig. 1. Inhibition of TbSPT2 causes growth arrest in T. brucei. (A) TbSPT2 expression in bloodstream (BF) and procyclic (PC) stage parasites was analyzed by northern blot hybridization. RNAi was induced in a procyclic cell line containing an integrated copy of the RNAi construct pZJM-TbSPT2 by the addition of 1 µg/ml Tet. TbSPT2 RNA levels were compared at day 1 post induction (TbSPT2 +Tet d1) with those in cells of the 29-13 cell line and in uninduced transfectants (TbSPT2–Tet d1). Ethidium bromide (EtBr)-stained gels are shown beneath the northern blots and the positions of molecular size standards are indicated (in kb). (B) The in vitro growth of the TbSPT2 RNAi cell line was assessed in the presence or absence of Tet induction and compared with that of the 29-13 line. Cultures of the three parasite lines were initiated in SDM79 medium at a density of 106 cells/ml and culture densities were determined daily for 6 days.





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