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Figure 2


Fig. 2. Sphingolipid biosynthesis is reduced by myriocin treatment or TbSPT2 RNAi. The T. brucei 29-13 cell line was grown in the absence or presence of myriocin (1.5 µM) for 1 day; TbSPT2 RNAi cells were grown for 3 days in the presence or absence of Tet and pulse-labeled with [3H]serine for 40 minutes. Lipids were extracted using the Blight-Dyer method and resolved by TLC and visualized by autoradiography. Each lane contains material from 5x108 cells. Positions of phosphatidylethanolamine (PE) and phosphatidylserine (PS) and ceramide standards are indicated.





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