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Figure 5


Fig. 5. LUMA topology explored by immunofluorescence. (A) HeLa cells were permeabilized with either digitonin or Triton X-100 and stained for LUMA. Tubulin or lamin B2 were visualized as controls. (B) Comparison of antibody-epitope accessibility of digitonin- and Triton-X-100-permeabilized cells overexpressing full-length LUMA or the LUMAgal mutant. LUMA (hydrophilic domain) and either the V5- or Myc-tag were visualized by immunofluorescence staining as indicated. Mock, vector control. (B) Comparison of antibody-epitope accessibility of digitonin- and Triton-X-100-permeabilized cells overexpressing LUMA truncation mutants LUMA SP 53-345, LUMA SP 53-309, and LUMA SP 53-200. Emerin and the Myc-tag were visualized by immunofluorescence staining. (B,C) For structural features of the constructs refer to Fig. 4B. Scale bars, 5 µm.





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