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Figure 8


Fig. 8. Downregulation of LUMA. HeLa cells were transfected with either a control vector expressing a control miRNA or the vector construct Hmi 48 expressing a miRNA designed to knockdown human LUMA mRNA. 72 hours after transfection, cells were fixed and subjected to immunofluorescence staining of LUMA, emerin and lamin A/C. Transfected cells are easily detected by GFP coexpression. Scale bars, 5 µm.





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