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Figure 2


Fig. 2. Normal lysosome function in the {Delta}ap3{delta} mutant. (A) Gelatin-SDS-PAGE analysis to detect the activities of mature lysosomal cysteine peptidase CPB (arrowhead) and metallopeptidases (asterisk). (B) Metabolic labelling and immunoprecipitation analysis assessing the maturation of CPB in the wild-type and {Delta}ap3{delta} cell lines. The mature CPB form is indicated by an arrowhead and the precursor forms by asterisks. Molecular masses are indicated on the right. (C) Labelling of the MVT-lysosome (arrowheads) in L. major {Delta}ap3{delta} and wild-type promastigotes with GFP-tagged syntaxins LmjF19.0120 and LmjF29.0070 (green signal) and endocytic tracer FM4-64 (red signal). n, nucleus; k, kinetoplast; scale bars, 10 µm. Corresponding differential interference contrast (DIC) images are shown as well.





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