QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Xenopus NEDD1 (XNEDD1) localizes to centrosomes and spindle microtubules. (Aa) Western blot of mitotic (M) and interphase (I) Xenopus egg extracts probed with antibodies raised against the C-terminal half of NEDD1 (amino acids 301-655). The position of molecular mass markers is indicated on the left. (b) Western blots of mitotic or interphase extracts treated with (+) alkaline phosphatase (AP) or control buffer (–). (B) Immunofluorescence micrographs of `cycled' microtubule asters (top panels) or spindles (bottom panels) induced by addition of sperm chromatin to egg extracts. Overlays show XNEDD1 in green and ${alpha}$ -tubulin in red. Scale bar, 10 µm. (C) Immunofluorescence micrographs of sperm tips (top panels) incubated in egg extract in the presence of nocodazole to prevent microtubule assembly, or sperm-induced asters (bottom panels) triple-labeled with antibodies against XNEDD1 or ${gamma}$ -tubulin, or stained for DNA, as indicated above the panels. Scale bars, 1 µm (top panels) and 10 µm (bottom panels). Overlays show XNEDD1 in green, ${gamma}$ -tubulin in red and DNA in blue. (D,E) Xenopus tissue culture cells at various cell cycle stages (indicated on the left) triple labeled for XNEDD1, ${alpha}$ -tubulin or ${gamma}$ -tubulin and DNA, as indicated above the panels. Overlays in D show XNEDD1 in green, ${alpha}$ -tubulin in red, DNA in blue. Overlays in E show ${gamma}$ -tubulin in green, XNEDD1 in red, DNA in blue. Scale bars, 10 µm.

Return to article