spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Xenopus NEDD1 (XNEDD1) depletion from Xenopus egg extract leads to disruption of microtubule structures. (A) Western blot analysis to determine the extent of XNEDD1 depletion. Relative amounts of mock-depleted and XNEDD1-depleted extract loaded are indicated above the lanes. (B) Schematic diagram of the constructs (XNEDD1-FL, XNEDD1-N and XNEDD1-C) used in this study. (C) Examples of sperm-induced asters assembled in mock-depleted (top panels) or XNEDD1-depleted extracts (all other panels). XNEDD1-depleted extracts were supplemented with buffer or full-length XNEDD1, XNEDD1-N or XNEDD1-C, as indicated. All extracts were supplemented with small amounts of rhodamine-tubulin to visualize microtubules (red in overlays). Signals in the rhodamine channel (left panels) or UV to visualize DNA (middle panels) were acquired separately and digitally overlaid. Scale bar, 10 µm. (D,E) Quantification of the (D) fluorescence intensity and (E) asters assembled around sperm centrioles for the experiments shown in C, expressed as percent of mock-depleted control. Error bars give the standard error (± s.e.). (F) Examples of spindles assembled in mock-depleted (top two rows of panels) or XNEDD1-depleted extracts (all other panels). Scale bar, 10 µm. (G) Quantification of the relative abundance of bipolar spindles, spindles with at least one disorganized pole and spindles with greatly reduced microtubule numbers in mock-depleted (light bars) and XNEDD1-depleted (dark bars) egg extracts. Error bars give the standard error (± s.e.).





Right arrow Return to article