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Fig. 6. Xenopus NEDD1 (XNEDD1) and ${gamma}$ -tubulin exist in distinct complexes. (A) Coomassie-Blue-stained gel of proteins that co-purify with control (NR, lane 1), XNEDD1 (lane 2) or ${gamma}$ -tubulin (lane 3) immunoprecipitations. Positions of ${gamma}$ TuRC subunits (Xgrips) are indicated on the right, positions of molecular mass markers are indicated on the left. Asterisks denote proteins unique to the XNEDD1 immunoprecipitation. (B) Western blots of 5-40% sucrose-gradient fractions probed for XNEDD1 (upper panel) or ${gamma}$ -tubulin (lower panel). Positions of molecular mass markers (ovalbumin (3.5 S), rabbit muscle aldolase (7.35 S), bovine liver catalase (11.3 S) and ferritin (17.6 S); run on a parallel gradient) are indicated below the blot. (C) Graphical representation of the western blots shown in (B). The intensity of western blot bands were measured and are shown as fraction of maximum for XNEDD1 and ${gamma}$ -tubulin, as indicated. The main peaks for XNEDD1 and ${gamma}$ -tubulin do not overlap. (D) Western blots of sucrose-gradient fractions of XNEDD1-depleted extracts probed for XNEDD1 (upper panel) or ${gamma}$ -tubulin (lower panel).

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