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Figure 1


Fig. 1. Sca-1 cell surface marker expression in adult murine skin. Epidermal whole mounts prepared from tail skin (A-F) and dorsal skin cryosections (G-J) were labeled with antibodies against the cell surface markers {alpha}6 integrin (A,G,E), Sca-1 (B,F,H) and CD34 (C,I). Fluorescent images were taken with a Zeiss LSM 510 confocal microscope. To visualize colocalization, A-C and G-I were merged to highlight UI cells in tail (D) or dorsal (J) skin. High-magnification images of A,B show continuous staining of {alpha}6 integrin (E) and Sca-1 (F) in the basal layer of the IFE. bg, bulge; IFD, infundibulum; IFE, interfollicular epidermis; sg, sebaceous gland. Scale bars: 50 µm (A-D and G-I); 10 µm (E,F).





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