spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Saline washing of mitochondria selectively suppresses autocatalysis of Bak conformation change. (A) Conventional (designated `unwashed') and saline-washed (`washed') mitochondria were prepared from the same cell homogenate. tBid-induced Bak conformation change (black symbols) and cytochrome c release (white symbols) were measured as in Fig. 6. The Bak data were best-fitted according to equation 1 (see Materials and Methods) to yield the f and c values given. These values were then used to obtain the best fits for the cytochrome c data according to equation 2 (see Materials and Methods) to give the curves shown and the a values given. (B) SDS-PAGE gels (Coomassie stained) and Bak immunoblots of the washed and unwashed mitochondrial preparations. Arrows show bands that were reduced in the washed preparation.





Right arrow Return to article