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Figure 2


Fig. 2. Composite of four near-field fluorescence measurements performed on a Cy5-IL2R{alpha}-labeled FT7.10 cell mapping the full distribution of the receptor on the membrane. The color coding of the image is the same as in Fig. 1. A compromise in image contrast accounts for the effect that the low-intensity spots in the middle of the cell are not clearly visible. The inset shows the central part of the cell with a different imaging contrast, revealing the presence of individual molecules (green and red spots corresponding to single dipole emission). Bar, 2 µm. The four independent NSOM images are shown in supplementary material Fig. S1.





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