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Figure 1


Fig. 1. Expression of active (pT555) PKC{iota} in the crypt-villus axis of the small intestine is synchronous with ezrin T567 phosphorylation. Frozen sections of mouse jejunum were stained with anti-T555-P (active) PKC{iota} antibody (A, green) and anti-T567-P ezrin antibody (C, red), or with the corresponding nonimmune IgGs (control, B, D). DNA was counterstained with DAPI (blue). (G, H) Phase-contrast images of the same sections overimposed with DAPI fluorescence. E, E', F show merged signals. E' is the field boxed in E showed at higher magnification. The images are shown with the bottom of the crypts to the left. Arrow in C, single isolated positive apical domain within a region of negative T567-P signal. Arrowheads show the lowest limit (earliest) of T567-P expression in the crypt. E' inset: higher magnification of the area blocked in E. Scale bar: 20 µm (A-H); 10 µm (E').





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