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Figure 3


Fig. 3. Conditional activation of p53 causes a decline in PI3K activity. (A) Western blot analyses demonstrate maximum AKT phosphorylation in OSEC2 cells after 60 minutes, and in IOSE 166h and IOSE 166a cells after 30 minutes, of stimulation with 10% FBS following serum starvation (0.5% FBS). Phosphorylated AKT (P-AKT) levels were lower at 39°C compared with at 34°C. (B) Western blots of OSEC2, IOSE 166h and IOSE 166a cells show that AKT-P levels decreased after a shift to 39°C, whereas total AKT (T-AKT) levels remained unchanged.





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