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Figure 2


Fig. 2. ORP3 overexpression induces cell-surface protrusions in HEK293 cells. HEK293 cells were transfected with N-terminally Xpress-tagged ORP3 plasmid constructs. At 20 hours post transfection, the cells were fixed and stained using anti-XpressTM antibodies. F-actin was visualized with OG488P. (A-C) Wild-type ORP3 (protrusions indicated by arrows), (D-F) ORP3mFFAT, (G-I) ORP3mPH2, (J-L) FR1(398-886), (M-O) FR6(1-555). Scale bars: 20 µm.





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