spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. ORP3 interacts with R-Ras. (A) HEK293 cells doubly transfected with ORP3/R-Ras(wt), ORP3/R-Ras(38V) or ORP3/R-Ras(43N) cDNA constructs were lysed in detergent-containing buffer, followed by removal of insoluble material by centrifugation. The insoluble pellets (P) and the soluble fractions (S) are depicted in the top panel. The S fractions were subjected to immunoprecipitation (IP) with anti-R-Ras or irrelevant control (Control IgG) rabbit antibodies. ORP3 and R-Ras in the immunocomplexes were visualized by western blotting (WB) using anti-XpressTM and anti-R-Ras antibodies, respectively. (B) HEK293 cells doubly transfected with the ORP3 cDNA and with R-Ras(wt), the constitutively active R-Ras(38V), or with the dominant inhibitory R-Ras(43N) and stained with anti-ORP3 and anti-R-Ras antibodies. (C) Untransfected HEK293 cells double-stained for endogenous ORP3 and R-Ras. The inset shows a magnified view of a protrusion end. The bottom panels display a similar specimen treated with the secondary antibody conjugates only, anti-rabbit Alexa Fluor 568 (A568R) and anti-goat-FITC (AGFITC), to demonstrate specificity of the staining patterns. Scale bars: 20 µm.





Right arrow Return to article