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Figure 1


Fig. 1. Characterisation of novel markers of NEP-A and NEP-B. (a) Affinity-purified antisera against XLAP2 were used to immunoblot Xenopus egg extracts (LSS) or cell extracts from Xenopus tissue culture cells (XTCs). Molecular mass standards are shown to the right of each blot. (b) Alternatively, XTC cells were prepared for immunofluorescence and stained with antibody against XLAP2 followed by TRITC-labelled goat anti-rabbit antibody and counter stained with DAPI. (c,d) NEP-A, NEP-B and membrane-free cytosol were resolved by 10% SDS-PAGE and either stained with Coomassie Blue (panel c) or immunoblotted (panel d) for the presence of CEL5C, CEL13A, LAP12, CEL1FF or XLAP2. M, molecular weight markers; MP1, NEP-A vesicles; MP2, NEP-B vesicles; CYT, membrane-free cytosol.





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