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Figure 4


Fig. 4. Inhibition of NE assembly by human emerin constructs. (a) NEP-A (labelled `-A'), NEP-B (labelled `-B') and cytosol were immunoblotted with antibodies against gp210, POM121. Molecular mass markers are indicated on the left-hand side. (b,c) Human emerin peptides 1-70 or 73-180 were added to Xenopus egg extracts at concentrations ranging from 0.5 µM to 8.0 µM. The ability of extracts to assemble sperm pronuclei in the presence of each peptide was assessed by either immunoblotting assays (b) or immunofluorescence assays (c). Here, mAb 4G12 was used to detect NEP-B and mAb CEL13A was used to detect NEP-A in immunofluorescence assays, whereas CEL1FF was used to detect NEP-B, and LAP12 was used to detect NEP-A in immunoblotting assays. CEL5C was used to detect total membranes. Bar, 10 µm.





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