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Figure 5


Fig. 5. Inhibition of NPC assembly by human emerin constructs. Human emerin peptides 1-70 (a,c) and 73-180 (b) were added to Xenopus egg extracts at concentrations between 0.5 and 8.0 µM. Alternatively, no peptides were added to extracts in order to measure normal assembly (labelled `control' in b,c). The effects of the peptides on NPC assembly were investigated by immunofluorescence using antibodies against FG-repeat nucleoporins (a,b) or Nup107 (c). Bars, 10 µm. (d) Alternatively, nuclei were assembled in extracts containing 4.0 µM peptide 73-180 (lane 1) or peptide 1-70 (lane 3). Nuclei were isolated and prepared for immunoblotting using antibodies against FG-repeat nucleoporins. Lane 2 contains material recovered from extracts containing no nuclei.





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