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Figure 7


Fig. 7. Depletion of NEP-A leads to assembly of nuclei missing components of the nucleoplasmic ring of NPCs. Fractionated extracts of Xenopus eggs were reconstituted with normal amounts of NEP-A and NEP-B (labelled `10') or with an amount such that NAP-A was depleted by a factor of ten relative to NEP-B (labelled `1'). Nuclei were prepared for either immunofluorescence with antibodies against FG-repeat nucleoporins 414 (a), Nup153 (b) and Nup214 (c) or immunoblotting (d) with antibodies against FG-repeat nucleoporins. Micrographs are presented as individual mono images in which the first image in each pair shows DAPI staining or as two-colour merged images. Bar, 10 µm.





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