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Figure 8


Fig. 8. Analysis by feSEM of NEs and NPCs assembled in the presence of emerin peptides. Sperm pronuclei were assembled in either the absence (labelled `control' in a,b) or presence of 4.0 µM emerin peptide 73-180 (c,d) or emerin peptide 1-70 (e,f). Samples were prepared for feSEM microscopy and examined at either low (a,c,e) or high (b,d,f) magnification. Ribosomes are indicated by arrows. Apparently intact fully formed NPCs are indicated by white circles, but these are rare in panel f, where more-abnormal structures predominate (black circles). Bars, 2000 nm (a,c,e); 100 nm (b,d,f).





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