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Figure 4


Fig. 4. Transport of the VSVG protein in a cell-free system is susceptible to HSP90-specific drugs and dependent on the interaction between the HSP90-TPR1 chaperone complex and VAP-33. (A) Intra-Golgi transport of VSVG protein is inhibited by geldanamycin, radicicol and macbecin compared with control assays. (B) Intra-Golgi transport can be inhibited by addition of the Tpr1unique fragment but not by bovine serum albumin (BSA) serving as a control. Quantified data are averages of three independent experiments. cpm, counts per minute; GlcNAc, N-acetylglucosamine. Bars, ± s.e.m.





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