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Fig. 5. The TPR1-specific antibody mAb292-200 inhibits intra-Golgi transport. 150 ng of antibody against TPR1 (mAb292-200) or an unrelated antibody against the Myc-tag (clone 9E10) serving as a control were added to 50 µl of the transport assay mixture and the transport reaction run for 1 hour, as described in Materials and Methods. The antibody against TPR1 but not the antibody against Myc results in decreased VSVG transport. Bars, ± s.e.m.