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Figure 2


Fig. 2. Expression of Sfrp1 in the anterior part of the embryo of medaka fish Oryzias latipes (olSfrp1) and chick (cSfrp1). (A-D) Panels show dorsal views of medaka fish (A,C) and chick (B,D) embryos hybridised in toto with digoxigenin-labelled species-specific probes against Sfrp. Gene transcripts accumulate in the most-anterior neural plate (black arrowheads in A) at mid-gastrula stages in both species. In both species expression is particularly abundant in the prospective eye field (orange arrowheads in A and B). In fish, expression is also observed in the future midbrain-hindbrain boundary (black arrowheads in A). During organogenesis (C,D) Sfrp1 expression is observed in the eye, otic vesicles, neural tube, somites and limb buds. hn, Hensen's node; St, embryonic stage, numbers indicate days. Scale bar in D: 50 µm for A, 125 µm for B, 100 µm for C, 500 µm for D.





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