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Figure 7


Fig. 7. Attaching a dilysine motif to GAT1-SSS supports its retrieval to the ER. (A) HEK293 cells were transfected with plasmids encoding CFP-tagged GAT1-SSS-KK. Images were acquired 24 hours later with a confocal microscope. (B,C) HEK293 cells expressing CFP-tagged GAT1-SSS-KK treated with 10 µg/ml BFA for 2 hours (B) and at 30 minutes after washout of BFA (C). (D,E) HEK293 cells co-expressing GAT1-SSS-KK and YFP-Golgi treated with 10 µg/ml BFA for 2 hours (D) and at 30 minutes after washout (E).





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