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Figure 8


Fig. 8. Biochemical analysis of interaction between PrPC and LRP1 on sensory neurons. (A) Immunoblot for PrP and Thy-1 of sequential immunoprecipitates (IP) of surface then internal antigen using antibody against PrPC or LRP1. The large arrow denotes the major 37-kDa di-glycosylated surface form of PrPC, the mid-size arrow shows monoglycosylated PrPC, and the small arrow shows non-glycosylated 23-kDa PrPC. PrP immunoblots are from the same gel, with duplicate samples run separately for Thy-1. (B) Immunoprecipitates for internal PrP or LRP1 (from part A) were diluted in endo H digestion buffer and either digested or not with the glycosidase, as indicated, before being immunoblotted for PrP. Arrows denote the relevant PrPC glycoforms as in A; the asterisk denotes the (slanted) band of endo H enzyme that binds antibodies nonspecifically.





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