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Figure 2


Fig. 2. Evolutionary conservation of ECM retention activity in trimeric and pentameric TSPs. (A) Schematic diagrams of Ciona TSPA, Drosophila TSP and COMP/TSP5. (B) Retention of the indicated TSPs in the ECM of COS-7 cells. Experimental conditions were as for Fig. 1B; TSPs were expressed with V5-epitope tags and detected with FITC-tagged V5 antibody. Scale bars: 20 µm. (C,D) Quantification of the relative expression (C) and ECM retention (D) of TSPs. Each column represents the mean of three to five experiments; bars represent s.e.m.





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