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Figure 7


Fig. 7. Bradykinin activates G{alpha}13 without affecting TJ assembly. (A,B) Calcium-switch experiments in G{alpha}12-MDCK cells (A, +dox; B, –dox) +/–bradykinin (100 µM) at t=0 as described in Fig. 6. (C) GST-TPR pull-downs from bradykinin-stimulated cells at the specified times after the calcium switch in G{alpha}12-MDCK cells +/–dox. Western blots were probed for G{alpha}12 and were reprobed for G{alpha}13 (designated by arrows). The bradykinin-stimulated cells (+/–dox) were analyzed on a single blot.





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