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Figure 2


Fig. 2. Reintroduction of human IKAP but not FD-IKAP rescues the plating efficiency, cell adhesion, spreading and migration phenotypes of IKAP-depleted MEFs. (A) Western blot analysis of IKAP-depleted MEFs (m1 and m2) with the reintroduction of wild-type IKAP and FD-IKAP. (B) RT-PCR analysis of IKBKAP in MEFs after IKAP depletion by shRNA and reintroduction of wild-type IKAP or FD-IKAP. Left, RT-PCR with oligonucleotides recognizing mouse IKBKAP; right, RT-PCR with oligonucleotides recognizing the 5' end of the human IKBKAP reintroduced to MEFs. (C) Plating efficiencies; (D) adhesion; (E) spreading; and (F) migration properties of the MEFs upon IKAP depletion (m1 and m2) and reintroduction of designated IKAP expression constructs together with corresponding empty vector (vector), scrambled (Scr) and protein expression vector pBabe controls were analyzed as in Fig. 1 (means ± s.d.; n=3; triplicates; **P<0.01; ***P<0.001). (G) Phase-contrast images of the designated wound-healing assays carried out as described in Fig. 1G.





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