spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Colocalization of VSVG-YFP and Sec31-CFP accumulation in ER exit site (ERES) membranes. (A) COS7 cells coexpressing VSVG-YFP (green) and Sec31-CFP (red) were treated as described in previous figures. Cells were transferred to the permissive temperature (34°C) and images were acquired at 2-minute intervals (see also supplementary material Movie 4). Scale bar: 10 µm. (B) Time-dependent increase in relative PFIVar of VSVG-YFP (green circles) and Sec31-CFP (red circles), calculated as described in the Materials and Methods, for the cell in A. Data were fitted to an exponential equation (y = aekx) (green and red lines for VSVG-YFP and Sec31-CFP, respectively). R2 values were 0.98 and 0.97, and k values were 6.4 and 1.65% per minute, for VSVG and Sec31, respectively.





Right arrow Return to article