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Figure 6


Fig. 6. Relationship between ARF1 and cargo-protein sorting to ER exit sites (ERESs). Confocal images of fixed COS7 cells coexpressing VSVG-YFP (green, A-B) and either the ARF1-CFP (A, red), or ARF1Q71L-CFP (B, red). Cells were transferred to ice for 20 minutes following a 24 hour incubation at the nonpermissive temperature (40°C). Nocodazole (1 µg/ml) was added, and the cells were returned for an additional 20 minutes to the nonpermissive temperature (40°C). After incubation at the permissive temperature (34°C) for the indicated times, cells were fixed by addition of 2% (v/v) formaldehyde and images were captured as described. Scale bars: 10 µm.





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