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Fig. 5. The bond strength of native OB-cadherins in differentiated myofibroblasts increases with increasing contact time. Differentiated myofibroblasts grown on tipless AFM cantilevers were put into contact with differentiated myofibroblasts grown in monolayer using a constant approach velocity (0.1 µm/second) and loading force (3 nN). (A) All obtained rupture forces are displayed as histograms (n
4000 per contact time), normalized for the total number of rupture events in every configuration and fitted with Gaussian curves. (B) With increasing contact time, the probability of obtaining three distinct force peaks increases; probability is calculated from the area under each individual Gaussian peak and divided by the total curve area (see supplementary material Fig. S2). (C) The average number of rupture jumps (±s.d.) preceding total cell detachment was determined in force-distance profiles for each contact time. (D) The total work needed to completely detach two differentiated myofibroblasts is displayed as a function of contact time (2-60 seconds).
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