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Fig. 7. Single-molecule bond strength of native cadherins in living myofibroblasts increases with increasing loading rate. (A) Differentiated myofibroblasts grown on tipless AFM cantilevers were put into contact for 2 seconds with myofibroblasts grown in a monolayer at 3 nN loading force and with approach velocities ranging from 0.1-1.0 µm/second (n>5000 per condition). All measured rupture forces are summarized in histograms that are fitted with Gaussian curves for approach velocities of 0.1, 0.2 and 1.0 µm/second, normalized to the total number of events. Dashed lines represent Gaussian fits of single peaks that are included in each total data set. (B) Overlaying all Gaussian fits obtained for each loading rate demonstrates a right shift in all Gaussian-fitted force peaks but no change in the number of peaks per histogram. (C) After fitting the data with Bell's model, force peak position is displayed as a function of the loading rate for each approach velocity.
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