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Figure 1


Fig. 1. Live imaging of endogenous grk mRNA. (A-C) grk*GFP expressed in the younger stages of mid-oogenesis. (A) Stage 7 shows posterior accumulation. (B) In early stage 8, there is often a lateral-cortical lining of grk*GFP on the side of the oocyte nucleus (asterisk). (C) During late 8, there is no longer a posterior lining of grk*GFP but instead the beginnings of an anterior ring with significant accumulation at the ventral anterior. (D,E) grk*RFP expressed in older stages of mid-oogenesis. From stages 9 to 10, there is a prominent dorsal-anterior cap and a strong reduction of grk*RFP at the ventral-anterior corner. (F) Control line expressing MCP-RFP alone. MCP-GFP and MCP-RFP have a nuclear localization sequence, and, as a consequence, when no grk mRNA with stem loops is present, the protein will enter the follicle cell nuclei (arrowhead) and nurse cell nuclei (arrow). Bars, 50 µm.





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