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Fig. 4. grk mRNA is a dynamic molecule. FRAP experiments were performed on grk^*GFP egg chambers at stages 6-9. (A-C) Images of grk^*GFP egg chambers at stages 6-9, with a small box representing the area that is bleached during a FRAP experiment. (D,E) Line graphs representing the average relative fluorescence of grk^*GFP during several FRAP experiments. (D) During stage 6, a defined area of fluorescent grk^*GFP at the oocyte posterior was bleached and about 70% was recovered. (E) grk^*GFP localized at the dorsal-anterior cap during late stage 9 was bleached and showed reduced recovery. (F) Bar graph representing the percentage of fluorescence recovery from stages 6 to late stage 9. There is a steady decrease in the amount of recovery as the oocyte progresses through development. (G) Bar graph representing the half-time of recovery (t_1/2) for stages 6 to late 9. Although recovery steadily decreased, the rate did not, suggesting there could be a similar mechanism occurring during stages 6 to early 9. A minimum of five FRAP experiments were performed for each data point. Bars represent the standard deviation; e, early; l, late.

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