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Figure 5


Fig. 5. grk mRNA recovery does not specifically involve depletion of nearby localized sources. (A,B) Images of stage 7 (A) and late stage 8 (B) egg chambers from flies expressing grk*GFP illustrating the region bleached during the FRAP experiment (black box), plus arrows pointing to the unbleached areas of grk*GFP monitored for fluorescence loss. (C,D) Line graphs representing average relative fluorescence of grk*GFP and two unbleached neighboring pools of grk*GFP during FRAP experiments. In panel C, the two posterior pools that surrounded the bleached region of grk*GFP during stage 7 show no dramatic decrease in fluorescence intensity. The pool on the right of the bleached region is represented by the dark gray line, whereas the pool on the left is the pale gray line. (D) Likewise, the surrounding sources of localized grk*GFP found at the dorsal-anterior cap during late stage 8 also displayed no measurable decrease while recovery occurred (right, dark gray; left, pale gray). This suggests that recovery is not due to nearby localized sources of grk*GFP.





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